![]() ![]() For analysis of small or very simple experiments, spreadsheet programs like Microsoft Excel are sufficient, and useful open-source tools exist for analysis and exploration of data from high throughput screens in general. The volume and richness of individual-cell data from large image-based screens is unprecedented and existing software is inadequate for the challenge of data analysis. This tool has been useful for extracting image-based measurements to score sophisticated screens, with many more in progress. ![]() We recently developed open-source image analysis software, CellProfiler, which measures a rich set of cellular features in images, such as size, shape, and staining patterns including intensity, texture, and colocalization. This capability is increasingly important given the ease now to systematically perturb cells with libraries of chemicals or gene-perturbing reagents like RNA interference or gene overexpression and collect hundreds of thousands of images of these cell samples. ![]() The scientific community has only begun to scratch the surface of computationally extracting the rich information visible in fluorescence microscopy images of cell samples. It is hard to understand how the resistance is being provided as endothelial cells are constantly exposed to oxidative stress and ROS during transport of oxygen in blood, but – still they are resistant to transformation.Visual analysis of cell samples has played a dominant role in the history of biology. TNFalpha receptors and death receptors, they are present in endothelial cells, but are they the major players in regulation of apoptosis in these cells? There are a lot of cutting edge papers about the role of TNFalpha in stimulation of metastasis and cancer progression, but endothelial cells are constantly exposed to TNFalpha which stimulates angiogenesis, but it does not make endothelial cells immortal, the cell division is still under control. Anyone looked at the telomeres length in HUVEC? Apoptosis pathway in endothelial cells: how it is different from the epithelial cell apoptosis? Which proteins of the apoptosis pathway are overrepresented in HUVEC? Proteomics analysis of endothelial cell profile – who is an expert in this area, please respond. What genes/mechanisms are responsible for this resistance? Anyone worked with angiosarcoma and tried to compare genetic profile of normal and malignant/transformed endothelial cells? If it is all about differentiation-related genes – which ones? VEGF is a mitogen for endothelial cells, but it stimulates angiogenesis, not cancer. ![]() However, endothelial cells are resistant to malignant transformation as angiosarcoma is a very rare type of cancer. In a cancer-affected organism, some of the endothelial cells are in tight contact with cancer cells and substances produced by malignant cells to stimulate growth and division. Endothelial cells are constantly exposed to concentrated mix of growth-stimulating hormones, cytokines, and metabolic agents during normal development. ![]()
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